Interaction between reactive oxygen species and hormones during the breaking of embryo dormancy in Sorbus pohuashanensis by exogenous nitric oxide

Abstract The breaking of dormancy mediated by reactive nitrogen species (RNS) is related to the accumulation oxygen (ROS) in germinating embryos but underlying mechanism unclear. objectives this study were: (1) explore relationship between RNS-mediated release and ROS Sorbus pohuashanensis ; and, (2) investigate relationships among germination time, metabolism, endogenous hormone synthesis. We studied effects exogenous nitric oxide (NO) donor sodium nitroprusside (SNP), NO scavenger (4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), abscisic acid (ABA), ethylene ethrel, receptor inhibitor 2,5-norbornadien (NBD) on embryo seedling growth. Embryos were released from pretreatment with or ethylene, which was increased biosynthesis decreased ABA levels. Breaking SNP levels hydrogen peroxide, glutathione, activities superoxide dismutase glutathione peroxidase, ABA, anions, malondialdehyde. These especially significant hypocotyls radicles. results demonstrate that can break S. inducing biosynthesis, signalling pathway closely antioxidant defence response.